Build with Toolkits

To achieve quick build times, we work with our clients to create a custom, modular DNA toolkit that encourages re-use of DNA parts.

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Custom modular DNA Toolkits

Build an entirely custom DNA toolkit to suit your organization's specific needs.

Designate functional categories for your different modular part types to standardize plasmid design across your organization

Take advantage of toolkits to enable efficient re-use of modular parts and facilitate onboarding of new team members

Streamline & Reuse

Streamline your plasmid designs and cloning using your DNA toolkit.

Use toolkits to support plasmid designs of different levels of complexity, from simple two-part assemblies to more advanced multi-part assemblies.

Use multiple assembly steps to achieve more complex plasmid designs containing operon or multi-gene configurations.

DNA Sourcing & Optimization

Vendor selection and ordering of synthesis-ready sequences.

Automatic redesign of difficult sequences to meet synthesis constraints (e.g., GC-rich regions, homopolymers).

Coordination with specialized synthesis providers for ordering of complex sequences.

Golden Gate cloning facilitates modularity, reuse, and speed.

Modular assembly using DNA toolkits

Share with us your generalized experimental workflow along with DNA sequences you'll be working with, and we will work with you to build a DNA toolkit that encourages efficient reuse of DNA parts in future projects while leveraging the benefits of Golden Gate cloning for efficient modular assembly of circular plasmids. Golden Gate cloning uses unique DNA overhangs to assemble DNA parts into plasmids in a defined order.

The DNA toolkit is a collection of parts that are each cloned into a standard entry vector, generating what we call a Basic Part plasmid; these Basic Part plasmids are sequence-verified clonal stocks that can be stored and propagated indefinitely for use in plasmid construction.

Building a DNA toolkit and reusing DNA parts has both economical and speed benefits: you save on DNA synthesis costs in the long run, and also reduce plasmid build times since the parts are ready on hand. Your DNA toolkit can be developed for applications that range from simple to complex. See the examples below for how DNA toolkits can support construction of plasmids of increasing complexity.

Example: TWO-part assemblies

A two-part assembly represents the simplest type of modular assembly. An example is cloning different ORFs into multiple possible expression vectors for e.g. protein expression or purification.

Example: SEVEN-part assemblies

More complex assemblies can be supported by the DNA toolkit, such as a 7-part assembly that might include the ability to permute multiple elements, including the promoter, terminator, selection markers, and origins of replication.

Example: multi-gene assemblies

Multi-gene assemblies are an example of a complex construct that can be assembled with a properly designed DNA toolkit. In this application, intermediate assembly plasmids are first constructed and then used to make the final assembly.

Introductory Trial

We are confident that our DNA cloning tools and services will bring increased productivity and organization to your research team.

We are offering a paid, no-commitment trial period to allow you to experience how our services can improve your research and to provide you with accurate sense of our quick build times.

You will receive

Access to our online Genie application for plasmid and cloning design, for an unlimited number of users

Direct line of communication to us via Slack for software technical support, design assistance, and coordination

Onboarding to Golden Gate cloning and modular DNA toolkit system

Assistance with design of a custom modular system, including up to 4 hours of meetings and consulting work

Thirty sequence-verified plasmids, split across two batches, e.g. 15 basic part plasmids and 15 assembly plasmids

Request a Trial

Introductory Trial

We are confident that our DNA cloning tools and services will bring increased productivity and organization to your research team.

We are offering a paid, no-commitment trial period to allow you to experience how our services can improve your research and to provide you with accurate sense of our quick build times.

You will receive

Access to our online Genie application for plasmid and cloning design, for an unlimited number of users

Direct line of communication to us via Slack for software technical support, design assistance, and coordination

Onboarding to Golden Gate cloning and modular DNA toolkit system

Assistance with design of a custom modular system, including up to 4 hours of meetings and consulting work

Thirty sequence-verified plasmids, split across two batches, e.g. 15 basic part plasmids and 15 assembly plasmids

Request a Trial

Introductory Trial

We are confident that our DNA cloning tools and services will bring increased productivity and organization to your research team.

We are offering a paid, no-commitment trial period to allow you to experience how our services can improve your research and to provide you with accurate sense of our quick build times.